Repression of lignin biosynthesis promotes cellulose accumulation and growth in transgenic trees.
Identifieur interne : 004863 ( Main/Exploration ); précédent : 004862; suivant : 004864Repression of lignin biosynthesis promotes cellulose accumulation and growth in transgenic trees.
Auteurs : W J Hu [États-Unis] ; S A Harding ; J. Lung ; J L Popko ; J. Ralph ; D D Stokke ; C J Tsai ; V L ChiangSource :
- Nature biotechnology [ 1087-0156 ] ; 1999.
Descripteurs français
- KwdFr :
- Arbres (croissance et développement), Arbres (génétique), Arbres (métabolisme), Cellulose (métabolisme), Lignine (antagonistes et inhibiteurs), Lignine (biosynthèse), Lignine (composition chimique), Phénotype (MeSH), Régulation de l'expression des gènes végétaux (MeSH), Régulation négative (MeSH), Spectroscopie par résonance magnétique (MeSH), Structure moléculaire (MeSH), Végétaux génétiquement modifiés (croissance et développement), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (métabolisme).
- MESH :
- antagonistes et inhibiteurs : Lignine.
- biosynthèse : Lignine.
- composition chimique : Lignine.
- croissance et développement : Arbres, Végétaux génétiquement modifiés.
- génétique : Arbres, Végétaux génétiquement modifiés.
- métabolisme : Arbres, Cellulose, Végétaux génétiquement modifiés.
- Phénotype, Régulation de l'expression des gènes végétaux, Régulation négative, Spectroscopie par résonance magnétique, Structure moléculaire.
English descriptors
- KwdEn :
- Cellulose (metabolism), Down-Regulation (MeSH), Gene Expression Regulation, Plant (MeSH), Lignin (antagonists & inhibitors), Lignin (biosynthesis), Lignin (chemistry), Magnetic Resonance Spectroscopy (MeSH), Molecular Structure (MeSH), Phenotype (MeSH), Plants, Genetically Modified (genetics), Plants, Genetically Modified (growth & development), Plants, Genetically Modified (metabolism), Trees (genetics), Trees (growth & development), Trees (metabolism).
- MESH :
- chemical , antagonists & inhibitors : Lignin.
- chemical , biosynthesis : Lignin.
- chemical , chemistry : Lignin.
- chemical , metabolism : Cellulose.
- genetics : Plants, Genetically Modified, Trees.
- growth & development : Plants, Genetically Modified, Trees.
- metabolism : Plants, Genetically Modified, Trees.
- Down-Regulation, Gene Expression Regulation, Plant, Magnetic Resonance Spectroscopy, Molecular Structure, Phenotype.
Abstract
Because lignin limits the use of wood for fiber, chemical, and energy production, strategies for its downregulation are of considerable interest. We have produced transgenic aspen (Populus tremuloides Michx.) trees in which expression of a lignin biosynthetic pathway gene Pt4CL1 encoding 4-coumarate:coenzyme A ligase (4CL) has been downregulated by antisense inhibition. Trees with suppressed Pt4CL1 expression exhibited up to a 45% reduction of lignin, but this was compensated for by a 15% increase in cellulose. As a result, the total lignin-cellulose mass remained essentially unchanged. Leaf, root, and stem growth were substantially enhanced, and structural integrity was maintained both at the cellular and whole-plant levels in the transgenic lines. Our results indicate that lignin and cellulose deposition could be regulated in a compensatory fashion, which may contribute to metabolic flexibility and a growth advantage to sustain the long-term structural integrity of woody perennials.
DOI: 10.1038/11758
PubMed: 10429249
Affiliations:
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Le document en format XML
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Ralph</name></author><author><name sortKey="Stokke, D D" sort="Stokke, D D" uniqKey="Stokke D" first="D D" last="Stokke">D D Stokke</name></author><author><name sortKey="Tsai, C J" sort="Tsai, C J" uniqKey="Tsai C" first="C J" last="Tsai">C J Tsai</name></author><author><name sortKey="Chiang, V L" sort="Chiang, V L" uniqKey="Chiang V" first="V L" last="Chiang">V L Chiang</name></author></analytic><series><title level="j">Nature biotechnology</title><idno type="ISSN">1087-0156</idno><imprint><date when="1999" type="published">1999</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cellulose (metabolism)</term><term>Down-Regulation (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Lignin (antagonists & inhibitors)</term><term>Lignin (biosynthesis)</term><term>Lignin (chemistry)</term><term>Magnetic Resonance Spectroscopy (MeSH)</term><term>Molecular Structure (MeSH)</term><term>Phenotype (MeSH)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (growth & development)</term><term>Plants, Genetically Modified (metabolism)</term><term>Trees (genetics)</term><term>Trees (growth & development)</term><term>Trees (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Arbres (croissance et développement)</term><term>Arbres (génétique)</term><term>Arbres (métabolisme)</term><term>Cellulose (métabolisme)</term><term>Lignine (antagonistes et inhibiteurs)</term><term>Lignine (biosynthèse)</term><term>Lignine (composition chimique)</term><term>Phénotype (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Régulation négative (MeSH)</term><term>Spectroscopie par résonance magnétique (MeSH)</term><term>Structure moléculaire (MeSH)</term><term>Végétaux génétiquement modifiés (croissance et développement)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cellulose</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Lignine</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Lignine</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Lignine</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Arbres</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants, Genetically Modified</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plants, Genetically Modified</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Arbres</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants, Genetically Modified</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Arbres</term><term>Cellulose</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Down-Regulation</term><term>Gene Expression Regulation, Plant</term><term>Magnetic Resonance Spectroscopy</term><term>Molecular Structure</term><term>Phenotype</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Phénotype</term><term>Régulation de l'expression des gènes végétaux</term><term>Régulation négative</term><term>Spectroscopie par résonance magnétique</term><term>Structure moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Because lignin limits the use of wood for fiber, chemical, and energy production, strategies for its downregulation are of considerable interest. We have produced transgenic aspen (Populus tremuloides Michx.) trees in which expression of a lignin biosynthetic pathway gene Pt4CL1 encoding 4-coumarate:coenzyme A ligase (4CL) has been downregulated by antisense inhibition. Trees with suppressed Pt4CL1 expression exhibited up to a 45% reduction of lignin, but this was compensated for by a 15% increase in cellulose. As a result, the total lignin-cellulose mass remained essentially unchanged. Leaf, root, and stem growth were substantially enhanced, and structural integrity was maintained both at the cellular and whole-plant levels in the transgenic lines. Our results indicate that lignin and cellulose deposition could be regulated in a compensatory fashion, which may contribute to metabolic flexibility and a growth advantage to sustain the long-term structural integrity of woody perennials.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">10429249</PMID><DateCompleted><Year>1999</Year><Month>09</Month><Day>16</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1087-0156</ISSN><JournalIssue CitedMedium="Print"><Volume>17</Volume><Issue>8</Issue><PubDate><Year>1999</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Nature biotechnology</Title><ISOAbbreviation>Nat Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Repression of lignin biosynthesis promotes cellulose accumulation and growth in transgenic trees.</ArticleTitle><Pagination><MedlinePgn>808-12</MedlinePgn></Pagination><Abstract><AbstractText>Because lignin limits the use of wood for fiber, chemical, and energy production, strategies for its downregulation are of considerable interest. We have produced transgenic aspen (Populus tremuloides Michx.) trees in which expression of a lignin biosynthetic pathway gene Pt4CL1 encoding 4-coumarate:coenzyme A ligase (4CL) has been downregulated by antisense inhibition. Trees with suppressed Pt4CL1 expression exhibited up to a 45% reduction of lignin, but this was compensated for by a 15% increase in cellulose. As a result, the total lignin-cellulose mass remained essentially unchanged. Leaf, root, and stem growth were substantially enhanced, and structural integrity was maintained both at the cellular and whole-plant levels in the transgenic lines. Our results indicate that lignin and cellulose deposition could be regulated in a compensatory fashion, which may contribute to metabolic flexibility and a growth advantage to sustain the long-term structural integrity of woody perennials.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>W J</ForeName><Initials>WJ</Initials><AffiliationInfo><Affiliation>Plant Biotechnology Research Center, School of Forestry and Wood Products, Michigan Technological University, Houghton, MI 49931, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Harding</LastName><ForeName>S A</ForeName><Initials>SA</Initials></Author><Author ValidYN="Y"><LastName>Lung</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Popko</LastName><ForeName>J L</ForeName><Initials>JL</Initials></Author><Author ValidYN="Y"><LastName>Ralph</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Stokke</LastName><ForeName>D D</ForeName><Initials>DD</Initials></Author><Author ValidYN="Y"><LastName>Tsai</LastName><ForeName>C J</ForeName><Initials>CJ</Initials></Author><Author ValidYN="Y"><LastName>Chiang</LastName><ForeName>V L</ForeName><Initials>VL</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Nat Biotechnol</MedlineTA><NlmUniqueID>9604648</NlmUniqueID><ISSNLinking>1087-0156</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015536" MajorTopicYN="N">Down-Regulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="Y">antagonists & inhibitors</QualifierName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009682" MajorTopicYN="N">Magnetic Resonance Spectroscopy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015394" MajorTopicYN="N">Molecular Structure</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1999</Year><Month>8</Month><Day>3</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2001</Year><Month>3</Month><Day>23</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1999</Year><Month>8</Month><Day>3</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">10429249</ArticleId><ArticleId IdType="doi">10.1038/11758</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Michigan</li></region></list><tree><noCountry><name sortKey="Chiang, V L" sort="Chiang, V L" uniqKey="Chiang V" first="V L" last="Chiang">V L Chiang</name><name sortKey="Harding, S A" sort="Harding, S A" uniqKey="Harding S" first="S A" last="Harding">S A Harding</name><name sortKey="Lung, J" sort="Lung, J" uniqKey="Lung J" first="J" last="Lung">J. Lung</name><name sortKey="Popko, J L" sort="Popko, J L" uniqKey="Popko J" first="J L" last="Popko">J L Popko</name><name sortKey="Ralph, J" sort="Ralph, J" uniqKey="Ralph J" first="J" last="Ralph">J. Ralph</name><name sortKey="Stokke, D D" sort="Stokke, D D" uniqKey="Stokke D" first="D D" last="Stokke">D D Stokke</name><name sortKey="Tsai, C J" sort="Tsai, C J" uniqKey="Tsai C" first="C J" last="Tsai">C J Tsai</name></noCountry><country name="États-Unis"><region name="Michigan"><name sortKey="Hu, W J" sort="Hu, W J" uniqKey="Hu W" first="W J" last="Hu">W J Hu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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